Journal: bioRxiv

Article Title: A novel mouse model of hypertensive emergency with multiorgan microvascular disease implicating the VEGFA/sFlt-1 balance

doi: 10.64898/2026.03.03.709451

Figure Lengend Snippet: A- Mice survival after hypertension onset (days after beginning of Ang II infusion). N= 20 C57BL6/J (B6J) and 129Sv (129) B- Mean daytime telemetry blood pressure N= 4 B6J and 129Sv. Values represent mean ± SEM. C- Mean nighttime telemetry blood pressure. N= 4 B6J and 129Sv. Values represent mean ± SEM.

Article Snippet: The hypertensive model was induced by subcutaneous infusion of angiotensin II (Ang II) (Alomone, GPA-100) at 1 μg/kg/min for 7 or 14 days, via osmotic minipumps (Alzet, model 1007D or 1002) in 10- to 16-week-old males.

Techniques:

Journal: bioRxiv

Article Title: A novel mouse model of hypertensive emergency with multiorgan microvascular disease implicating the VEGFA/sFlt-1 balance

doi: 10.64898/2026.03.03.709451

Figure Lengend Snippet: A- In vivo imaging (microm) performed on 129Sv (129) and C57BL6/J (B6J) mice 8 days after angiotensin-2 pump implantation shows haemorrhage (arrows) and hard exudates (asterisks). Haemorrhagic spots are only found on the 129Sv eye fundus and density of hard exudates is increased in 129 mice compared to B6J mice. B-Haemorrhagic spots (arrow heads) in retinas at day 7 of hypertensive challenge and associated quantification. Values represent mean ± SEM of 5 mice per group. * p < 0.05. C- Subretinal swelling assessed by OCT at day 7 of hypertensive challenge and associated quantification. The white arrow shows retinal detachment. D- Retinal capillary density assessed at day 7 of hypertensive challenge. Representative images of the retina vascular network stained with lectin antibody. Values represent mean ± SEM of 4 B6J mice and 8 129 mice. *** p < 0.001.

Article Snippet: The hypertensive model was induced by subcutaneous infusion of angiotensin II (Ang II) (Alomone, GPA-100) at 1 μg/kg/min for 7 or 14 days, via osmotic minipumps (Alzet, model 1007D or 1002) in 10- to 16-week-old males.

Techniques: In Vivo Imaging, Staining

Journal: bioRxiv

Article Title: A novel mouse model of hypertensive emergency with multiorgan microvascular disease implicating the VEGFA/sFlt-1 balance

doi: 10.64898/2026.03.03.709451

Figure Lengend Snippet: Typical ECG changes seen in SV129 mice treated for 2 weeks with angiotensin II (n = 5-7 mice/group. A. episode of atrial flutter B. atrial fibrillation. C. ventricular ectopy seen in the Ang II group; D. short run of ventricular tachycardia; E. sustained ventricular tachycardia degenerating in ventricular fibrillation and cardiac death.

Article Snippet: The hypertensive model was induced by subcutaneous infusion of angiotensin II (Ang II) (Alomone, GPA-100) at 1 μg/kg/min for 7 or 14 days, via osmotic minipumps (Alzet, model 1007D or 1002) in 10- to 16-week-old males.

Techniques:

Journal: Purinergic Signalling

Article Title: Immunolocalisation of P2Y receptors in the rat eye

doi: 10.1007/s11302-004-5072-5

Figure Lengend Snippet: Distribution of P2Y receptors in the anterior segment of the eye.

Article Snippet: Antibodies against the P2Y receptors were purchased from Alomone Labs (Israel), except P2Y 11 which was a gift from Dr D. Cousens (Glaxo-Smithkline).

Techniques:

Journal: Purinergic Signalling

Article Title: Immunolocalisation of P2Y receptors in the rat eye

doi: 10.1007/s11302-004-5072-5

Figure Lengend Snippet: Presence of P2Y 4 receptors in the iris. Iris sections presented positive staining to P2Y 4 receptor antibody (in red). Vimentin (to label intermediate filaments) or synaptophysin (nerve terminals) in green demonstrated some co-localisation between P2Y 4 receptors and the other markers.

Article Snippet: Antibodies against the P2Y receptors were purchased from Alomone Labs (Israel), except P2Y 11 which was a gift from Dr D. Cousens (Glaxo-Smithkline).

Techniques: Staining

Journal: Purinergic Signalling

Article Title: Immunolocalisation of P2Y receptors in the rat eye

doi: 10.1007/s11302-004-5072-5

Figure Lengend Snippet: P2Y receptors in the ciliary body and related areas. Positive labelling to P2Y 1 , P2Y 2 (upper panel), P2Y 4 (mid panel) and P2Y 6 (lower panel) were observed in the ciliary body (in red). It is noteworthy that the distribution of the receptor depends on the particular P2Y subtype. P2Y 2 labelled all the ciliary body while P2Y 6 mainly labelled the stromal area. Vimentin (in green) labels the non-pigmented epithelial cells as well as the lens capsule and helps to understand the distribution of some of the P2Y receptors.

Article Snippet: Antibodies against the P2Y receptors were purchased from Alomone Labs (Israel), except P2Y 11 which was a gift from Dr D. Cousens (Glaxo-Smithkline).

Techniques:

Journal: Purinergic Signalling

Article Title: Immunolocalisation of P2Y receptors in the rat eye

doi: 10.1007/s11302-004-5072-5

Figure Lengend Snippet: Distribution of P2Y receptors in the retina.

Article Snippet: Antibodies against the P2Y receptors were purchased from Alomone Labs (Israel), except P2Y 11 which was a gift from Dr D. Cousens (Glaxo-Smithkline).

Techniques:

Journal: Purinergic Signalling

Article Title: Immunolocalisation of P2Y receptors in the rat eye

doi: 10.1007/s11302-004-5072-5

Figure Lengend Snippet: Presence of P2Y 2 and P2Y 11 in the RPE. Labelling to the P2Y 2 receptor was obtained in the retinal pigmented epithelium (upper pictures). Combination with synaptophysin labelling permits a better identification of the retinal areas. P2Y 11 receptors were also present in the RPE as observed in the three lower pictures. Scale bar, 10 µm.

Article Snippet: Antibodies against the P2Y receptors were purchased from Alomone Labs (Israel), except P2Y 11 which was a gift from Dr D. Cousens (Glaxo-Smithkline).

Techniques:

Journal: Purinergic Signalling

Article Title: Immunolocalisation of P2Y receptors in the rat eye

doi: 10.1007/s11302-004-5072-5

Figure Lengend Snippet: Distribution of P2Y 4 receptors in the retina. (A) Schematic diagram showing the main cells in the retina, the synapses, and how they are related. (B) The presence of P2Y 4 receptors in the retina. Upper panel, labelling with the P2Y 4 receptor antibody presenting positive staining in the external segments of photoreceptors (OS), outer plexiform layer (OPL) and ganglion cell layer (GCL). Mid panel, synaptophysin staining labelling the synaptic layer outer plexifom layer (OPL) and inner plexiform layer (IPL). Lower panel, combination of the upper and mid panels.

Article Snippet: Antibodies against the P2Y receptors were purchased from Alomone Labs (Israel), except P2Y 11 which was a gift from Dr D. Cousens (Glaxo-Smithkline).

Techniques: Staining

Journal: Purinergic Signalling

Article Title: Immunolocalisation of P2Y receptors in the rat eye

doi: 10.1007/s11302-004-5072-5

Figure Lengend Snippet: Study on the co-localisation of P2Y 4 and P2Y 6 receptors with vimentin (Müller cells). Upper pictures represent the labelling with the P2Y 4 receptor antibody (upper), with the vimentin (mid) antibody and a combination of both (lower). Lower pictures represent the labelling of P2Y 6 receptors (upper), vimentin (mid) and a combination of both (lower). Scale bar, 10 µ.

Article Snippet: Antibodies against the P2Y receptors were purchased from Alomone Labs (Israel), except P2Y 11 which was a gift from Dr D. Cousens (Glaxo-Smithkline).

Techniques:

Journal: Purinergic Signalling

Article Title: Immunolocalisation of P2Y receptors in the rat eye

doi: 10.1007/s11302-004-5072-5

Figure Lengend Snippet: Western blot for the P2Y receptors in corneal extracts. Analysis of the P2Y receptors present in the cornea by Western blot analysis following the protocol described in Materials and methods. The molecular weights of the identified bands fit quite well with those described in the literature.

Article Snippet: Antibodies against the P2Y receptors were purchased from Alomone Labs (Israel), except P2Y 11 which was a gift from Dr D. Cousens (Glaxo-Smithkline).

Techniques: Western Blot